Isolation and characterization of malaria PfHRP2 specific V_NAR antibody fragments from immunized shark phage display library.

ABSTRACT:

Background

Malaria rapid diagnostic tests (RDTs) represent an important antibody based immunoassay platform. Unfortunately, conventional monoclonal antibodies are subject to degradation shortening shelf lives of RDTs. The variable region of the receptor (V_NAR) from shark has a potential as alternative to monoclonal antibodies in RDTs due to high thermal stability.

Methods

In this study, new binders derived from shark V_NAR domains library were investigated. Following immunization of a wobbegong shark (Orectolobus ornatus) with three recombinant malaria biomarker proteins (PfHRP2, PfpLDH and Pvaldolase), a single domain antibody (sdAb) library was constructed from splenocytes. Target-specific V_NAR phage were isolated by panning. One specific cl

SUBMITTER: Leow CH 

PROVIDER: S-EPMC6201582 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature