Genetically Encoded Catalytic Hairpin Assembly for Sensitive RNA Imaging in Live Cells.

ABSTRACT: DNA and RNA nanotechnology has been used for the development of dynamic molecular devices. In particular, programmable enzyme-free nucleic acid circuits, such as catalytic hairpin assembly, have been demonstrated as useful tools for bioanalysis and to scale up system complexity to an extent beyond current cellular genetic circuits. However, the intracellular functions of most synthetic nucleic acid circuits have been hindered by challenges in the biological delivery and degradation. On the other hand, genetically encoded and transcribed RNA circuits emerge as alternative powerful tools for long-term embedded cellular analysis and regulation. Herein, we reported a genetically encoded RNA-based catalytic hairpin assembly circuit for sensitive RNA imaging inside living cells. The split versio

SUBMITTER: Karunanayake Mudiyanselage APKK 

PROVIDER: S-EPMC6201751 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature