MiR-216a-5p inhibits malignant progression in small cell lung cancer: involvement of the Bcl-2 family proteins.

ABSTRACT:

Objective

microRNAs are regulatory molecules regarded as important in the pathogenesis of different types of tumors. microRNA-216a (miR-216a-5p) has been identified as a tumor suppressor in multiple malignancies. However, the role of miR-216a-5p in the pathogenesis of small cell lung cancer (SCLC) remains obscure. The objective of this study was to investigate the role of the miR-216a-5p/Bcl-2 axis in SCLC pathogenesis.

Materials and methods

All the experimental methods used were as follows: microarray analysis, cell culture, transient, and stable gene transfection; real-time fluorescence PCR; Western blot; flow cytometry for cell cycle analysis; in vitro proliferation assay; in vitro wound healing experiment; in vivo xenograft model in nude mice; and dual luciferase reporte

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6201844 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature