Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging.

ABSTRACT: Modern molecular imaging techniques have greatly improved tumor detection and post-treatment follow-up of cancer patients. In this context, antibody-based imaging is rapidly becoming the gold standard, since it combines the unique specificity of antibodies with the sensitivity of the different imaging technologies. The aim of this study was to generate and characterize antibodies in single chain Fragment variable (scFv) format directed to an emerging cancer biomarker, the human ether-à-go-go-related gene-1 (hERG1) potassium channel, and to obtain a proof of concept for their potential use for in vivo molecular imaging. The anti-hERG1scFv was generated from a full length monoclonal antibody and then mutagenized, substituting a Phenylalanine residue in the third framework of th

SUBMITTER: Duranti C 

PROVIDER: S-EPMC6201861 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature