Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling.

ABSTRACT: Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.

SUBMITTER: Myers SA 

PROVIDER: S-EPMC6202184 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature