Rerouting of NADPH synthetic pathways for increased protopanaxadiol production in Saccharomyces cerevisiae.

ABSTRACT: Ginseng (Panax ginseng) and its bioactive components, ginsenosides, are popular medicinal herbal products, exhibiting various pharmacological effects. Despite their advocated use for medication, the long cultivation periods of ginseng roots and their low ginsenoside content prevent mass production of this compound. Yeast Saccharomyces cerevisiae was engineered for production of protopanaxadiol (PPD), a type of aglycone characterizing ginsenoside. PPD-producing yeast cell factory was further engineered by obtaining a balance between enzyme expressions and altering cofactor availability. Different combinations of promoters (P_GPD, P_CCW12, and P_ADH2) were utilized to construct the PPD biosynthetic pathway. Rerouting the redox metabolism to improve NADPH availab

SUBMITTER: Kim JE 

PROVIDER: S-EPMC6202386 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature