In Vivo Two-Photon Voltage Imaging with Sulfonated Rhodamine Dyes.

ABSTRACT: Optical methods that rely on fluorescence for mapping changes in neuronal membrane potential in the brains of awake animals provide a powerful way to interrogate the activity of neurons that underlie neural computations ranging from sensation and perception to learning and memory. To achieve this goal, fluorescent indicators should be bright, highly sensitive to small changes in membrane potential, nontoxic, and excitable with infrared light. We report a new class of fluorescent, voltage-sensitive dyes: sulfonated rhodamine voltage reporters (sRhoVR), synthetic fluorophores with high voltage sensitivity, excellent two-photon performance, and compatibility in intact mouse brains. sRhoVR dyes are based on a tetramethyl rhodamine fluorophore coupled to a phenylenevinylene molecular wire/dieth

SUBMITTER: Kulkarni RU 

PROVIDER: S-EPMC6202643 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature