Green Tea Catechin Dramatically Promotes RNAi Mediated by Low-Molecular-Weight Polymers.

ABSTRACT: Cytosolic delivery is the major challenge that limits the clinical translation of siRNA-based therapeutics. Although thousands of polymers have been developed for siRNA delivery, the efficiency-toxicity correlation is unsatisfactory. Here, we report a facile strategy to fabricate core-shell-structured nanoparticles with robust siRNA delivery efficiency. The nanoparticle is prepared by entropy-driven complexation of siRNA with a green tea catechin to yield a negatively charged core, followed by coating low-molecular-weight polymers to form the shell. This supramolecular strategy facilitates the polymers condensing siRNA into uniform nanoparticles. The nanoparticle specifically down-regulates target genes in vitro and in vivo, and efficiently attenuates chronic intestinal infla

SUBMITTER: Shen W 

PROVIDER: S-EPMC6202644 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature