Bovine leukemia virus long terminal repeat variability: identification of single nucleotide polymorphisms in regulatory sequences.

ABSTRACT:

Background

Limited data are available on the incidence of variations in nucleotide sequences of long terminal repeat (LTR) regions of Bovine Leukemia Virus (BLV). Consequently, the possible impact of SNPs on BLV LTR function are poorly elucidated. Thus, a detailed and representative study of full-length LTR sequences obtained from sixty-four BLV isolates from different geographical regions of Poland, Moldova, Croatia, Ukraine and Russia were analyzed for their genetic variability.

Methods

Overlap extension PCR, sequencing and Bayesian phylogenetic reconstruction of LTR sequences were performed. These analyses were followed by detailed sequence comparison, estimation of genetic heterogeneity and identification of transcription factor binding site (TFBS) modifications.

Resu

SUBMITTER: Pluta A 

PROVIDER: S-EPMC6202831 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature