ITRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals.

ABSTRACT:

Background

Human saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with aging of patients with dental caries. Here, we applied isobaric tags for relative and absolute quantitation (iTRAQ) in combination with multiple reaction monitoring mass spectrometry (MRM-MS) to characterize the salivary proteome profiles of subjects of different ages, presenting with and without caries, with the aim of identifying age-related biomarkers for dental caries.

Methods

Unstimulated whole saliva samples were collected from 40 caries-free and caries-susceptible young adults and elderly individuals. Salivary proteins were extracted, reduced, alkylat

SUBMITTER: Wang K 

PROVIDER: S-EPMC6202833 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature