Targeted deletion of Insm2 in mice result in reduced insulin secretion and glucose intolerance.

ABSTRACT:

Background

Neurogenin3 (Ngn3) and neurogenic differentiation 1 (NeuroD1), two crucial transcriptional factors involved in human diabetes (OMIM: 601724) and islet development, have been previously found to directly target to the E-boxes of the insulinoma-associated 2 (Insm2) gene promoter, thereby activating the expression of Insm2 in insulin-secretion cells. However, little is known about the function of Insm2 in pancreatic islets and glucose metabolisms.

Methods

Homozygous Insm2^-/- mice were generated by using the CRISPR-Cas9 method. Glucose-stimulated insulin secretion and islet morphology were analyzed by ELISA and immunostainings. Expression levels of Insm2-associated molecules were measured using quantitative RT-PCR and Western blots.

Results

Fasting b

SUBMITTER: Wang L 

PROVIDER: S-EPMC6202866 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature