Integrative analysis of microRNA and mRNA expression profiles in fetal rat model with anorectal malformation.

ABSTRACT:

Background

Anorectal malformations (ARMs) are the most common congenital malformation of the gut, and regulated by multiple signal transduction pathways. The microRNA (miRNA) expression profiles and their biologial functions in anorectal malformations (ARMs) remain unclear. The aim of our study was to evaluate miRNA and mRNA expression profiles in the ARM rats.

Methods and materials

ARM was induced with ethylenethiourea (ETU) on gestational day 10. Cesarean deliveries were performed to harvest the embryos on gestional day 20. For the extraction of total RNA, 1 cm terminal hindgut samples were obtained from three fetal rats that had similar weights. The microarrays and quantitative RT-PCR analysis were conducted to evaluate the miRNA and mRNA expression profiles in normal fet

SUBMITTER: Xiao H 

PROVIDER: S-EPMC6203938 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature