Fluorescent labeling of endogenous platelets for intravital microscopy: Effects on platelet function.

ABSTRACT:

Objective

Monitoring endogenous platelets during intravital microscopy often involves two approaches: fluorescently labeled antibodies or genetic models of platelet-specific fluorescent protein expression. Due to limited data available on platelet functional changes induced by these methods, we compared functional effects of these methods on platelets.

Methods

Platelet aggregation to collagen and thrombin, and collagen matrix-mediated platelet adhesion/aggregation under flow were tested. We assessed platelets from mice expressing EYFP on platelets (Cre(+)), littermate controls (Cre(-)), C57BL/6 mice, and platelets from vehicle control and x-488 treatment. We utilized intravital microscopy to monitor platelets in vivo using Cre(+) mice and x-488 treatment.

Results

Both

SUBMITTER: Da Q 

PROVIDER: S-EPMC6204114 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature