Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch.

ABSTRACT: Polymyxin E or colistin, produced by Paenibacillus polymyxa, is an important antibiotic against Gram-negative pathogens. The objective of this study is to evaluate the effect of starch in fermentation medium on colistin biosynthesis in P. polymyxa. The results indicated that replacement of glucose by starch stimulated colistin production and biosynthesis rate. Overall, the stimulation extent was starch concentration-dependent. As expected, addition of starch induced the expression of amyE encoding amylase and increased amylase activity in fermentation solution. Additionally, replacement of glucose by starch resulted in residue reducing sugar and pH of fermentation mixture low relative to glucose as the sole sugar source. At the molecular level, it was found that replac

SUBMITTER: Yu Z 

PROVIDER: S-EPMC6204185 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature