Effect of lignin fractions isolated from different biomass sources on cellulose oxidation by fungal lytic polysaccharide monooxygenases.

ABSTRACT:

Background

Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that oxidatively cleave recalcitrant lignocellulose in the presence of oxygen or hydrogen peroxide as co-substrate and a reducing agent as electron donor. One of the possible systems that provide electrons to the LPMOs active site and promote the polysaccharide degradation involves the mediation of phenolic agents, such as lignin, low-molecular-weight lignin-derived compounds and other plant phenols. In the present work, the interaction of the bulk insoluble lignin fraction extracted from pretreated biomass with LPMOs and the ability to provide electrons to the active site of the enzymes is studied.

Results

The catalytic efficiency of three LPMOs, namely MtLPMO9 with C1/C4 regioselecti

SUBMITTER: Muraleedharan MN 

PROVIDER: S-EPMC6204277 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature