Oxidative stress-induced dysregulation of excitation-contraction coupling contributes to muscle weakness.

ABSTRACT:

Background

We have previously shown that the deletion of the superoxide scavenger, CuZn superoxide dismutase, in mice (Sod1^-/- mice) results in increased oxidative stress and an accelerated loss of skeletal muscle mass and force that mirror the changes seen in old control mice. The goal of this study is to define the effect of oxidative stress and ageing on muscle weakness and the Excitation Contraction (EC) coupling machinery in age-matched adult (8-10 months) wild-type (WT) and Sod1^-/- mice in comparison with old (25-28 months) WT mice.

Methods

In vitro contractile assays were used to measure muscle contractile parameters. The activity of the sarcoplasmic reticulum Ca²⁺ ATPase (SERCA) pump was measured using an NADH-linked enzyme assay. Imm

SUBMITTER: Qaisar R 

PROVIDER: S-EPMC6204588 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature