Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America.

ABSTRACT: BACKGROUND Human trichinellosis is a foodborne parasitic zoonotic disease caused by ingestion of raw or undercooked meat infected with nematode larvae of the genus Trichinella. In the USA, sporadic cases and outbreaks caused by the consumption of wild game meat infected with Trichinella have been reported. The current methods for diagnosis such as serology and microscopy are not specific, may result in false negative results, and cannot differentiate encapsulated Trichinella larvae to species level. The molecular protocols currently available for the differentiation of all encapsulate Trichinella species prevalent in North America have some limitations such as the inability to identify and resolve the presence of several Trichinella species in a single test. OBJECTIVES/METHODS In this stud

SUBMITTER: Almeida M 

PROVIDER: S-EPMC6204614 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature