Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli.

ABSTRACT: The widespread use of monoclonal antibodies (mAbs) as a platform for therapeutic drug development in the pharmaceutical industry has led to an increased interest in robust experimental approaches for assessment of mAb structure, stability and dynamics. The ability to enrich proteins with stable isotopes is a prerequisite for the in-depth application of many structural and biophysical methods, including nuclear magnetic resonance (NMR), small angle neutron scattering, neutron reflectometry, and quantitative mass spectrometry. While mAbs can typically be produced with very high yields using mammalian cell expression, stable isotope labeling using cell culture is expensive and often impractical. The most common and cost-efficient approach to label proteins is to express proteins in Escherichi

SUBMITTER: Reddy PT 

PROVIDER: S-EPMC6204800 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature