Intracellular displacement of p53 using transactivation domain (p53 TAD) specific nanobodies.

ABSTRACT: The tumor suppressor p53 is of crucial importance in the prevention of cellular transformation. In the presence of cellular stress signals, the negative feedback loop between p53 and Mdm2, its main negative regulator, is disrupted, which results in the activation and stabilization of p53. Via a complex interplay between both transcription-dependent and - independent functions of p53, the cell will go through transient cell cycle arrest, cellular senescence or apoptosis. However, it remains difficult to completely fathom the mechanisms behind p53 regulation and its responses, considering the presence of multiple layers involved in fine-tuning them. In order to take the next step forward, novel research tools are urgently needed. We have developed single-domain antibodies, also known as nano

SUBMITTER: Steels A 

PROVIDER: S-EPMC6204812 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature