Data-independent oxonium ion profiling of multi-glycosylated biotherapeutics.

ABSTRACT: The characterization of glycosylation is required for many protein therapeutics. The emergence of antibody and antibody-like molecules with multiple glycan attachment sites has rendered glycan analysis increasingly more complicated. Reliance on site-specific glycopeptide analysis is therefore necessary to fully analyze multi-glycosylated biotherapeutics. Established glycopeptide methodologies have generally utilized a priori knowledge of the glycosylation states of the investigated protein(s), database searching of results generated from data-dependent liquid chromatography-tandem mass spectrometry workflows, and extracted ion quantitation of the individual identified species. However, the inherent complexity of glycosylation makes predicting all glycoforms on all glycosylation sites extre

SUBMITTER: Madsen JA 

PROVIDER: S-EPMC6204843 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature