Endogenous insertion of non-native metalloporphyrins into human membrane cytochrome P450 enzymes.

ABSTRACT: Human cytochrome P450 enzymes are membrane-bound heme-containing monooxygenases. As is the case for many heme-containing enzymes, substitution of the metal in the center of the heme can be useful for mechanistic and structural studies of P450 enzymes. For many heme proteins, the iron protoporphyrin prosthetic group can be extracted and replaced with protoporphyrin containing another metal, but human membrane P450 enzymes are not stable enough for this approach. The method reported herein was developed to endogenously produce human membrane P450 proteins with a nonnative metal in the heme. This approach involved coexpression of the P450 of interest, a heme uptake system, and a chaperone in Escherichia coli growing in iron-depleted minimal medium supplemented with the desired trans-me

SUBMITTER: Yadav R 

PROVIDER: S-EPMC6204904 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature