Highly specific and selective anti-pS396-tau antibody C10.2 targets seeding-competent tau.

ABSTRACT:

Introduction

The abnormal hyperphosphorylation of the microtubule-associated protein tau plays a crucial role in neurodegeneration in Alzheimer's disease (AD) and other tauopathies.

Methods

Highly specific and selective anti-pS396-tau antibodies have been generated using peptide immunization with screening against pathologic hyperphosphorylated tau from rTg4510 mouse and AD brains and selection in in vitro and in vivo tau seeding assays.

Results

The antibody C10.2 bound specifically to pS396-tau with an IC₅₀ of 104 pM and detected preferentially hyperphosphorylated tau aggregates from AD brain with an IC₅₀ of 1.2 nM. C10.2 significantly reduced tau seeding of P301L human tau in HEK293 cells, murine cortical neurons, and mice. AD brain extracts de

SUBMITTER: Rosenqvist N 

PROVIDER: S-EPMC6205114 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature