Cryo-ET reveals the macromolecular reorganization of S. pombe mitotic chromosomes in vivo.

ABSTRACT: Chromosomes condense during mitosis in most eukaryotes. This transformation involves rearrangements at the nucleosome level and has consequences for transcription. Here, we use cryo-electron tomography (cryo-ET) to determine the 3D arrangement of nuclear macromolecular complexes, including nucleosomes, in frozen-hydrated Schizosaccharomyces pombe cells. Using 3D classification analysis, we did not find evidence that nucleosomes resembling the crystal structure are abundant. This observation and those from other groups support the notion that a subset of fission yeast nucleosomes may be partially unwrapped in vivo. In both interphase and mitotic cells, there is also no evidence of monolithic structures the size of Hi-C domains. The chromatin is mingled with two features: pockets, whi

SUBMITTER: Cai S 

PROVIDER: S-EPMC6205422 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature