Characterizing posttranslational modifications in prokaryotic metabolism using a multiscale workflow.

ABSTRACT: Understanding the complex interactions of protein posttranslational modifications (PTMs) represents a major challenge in metabolic engineering, synthetic biology, and the biomedical sciences. Here, we present a workflow that integrates multiplex automated genome editing (MAGE), genome-scale metabolic modeling, and atomistic molecular dynamics to study the effects of PTMs on metabolic enzymes and microbial fitness. This workflow incorporates complementary approaches across scientific disciplines; provides molecular insight into how PTMs influence cellular fitness during nutrient shifts; and demonstrates how mechanistic details of PTMs can be explored at different biological scales. As a proof of concept, we present a global analysis of PTMs on enzymes in the metabolic network of Escheric

SUBMITTER: Brunk E 

PROVIDER: S-EPMC6205427 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature