Highly efficient siRNA transfection in macrophages using apoptotic body-mimic Ca-PS lipopolyplex.

ABSTRACT:

Background

The discovery and development of RNA interference has made a tremendous contribution to the biochemical and biomedical field. However, liposomal transfection protocols to deliver siRNAs to certain types of cells, eg, immune cells, are not viable due to exceedingly low transfection efficiency. While viral delivery and electroporation are two widely adopted approaches to transfect immune cells, they are associated with certain drawbacks such as complexity of preparation, biosafety issues, and high cytotoxicity. We believe amendments can be made to liposomal formulas and protocols to achieve a highly efficient knockdown of genes by liposome-loaded siRNAs.

Aim

The aim of this study was to use the apoptotic-mimic Ca-PS lipopolyplex to achieve highly efficient siRNA kno

SUBMITTER: Lai Y 

PROVIDER: S-EPMC6205523 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature