Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot.

ABSTRACT:

Background

Meiotic recombination hotspots control the frequency and distribution of Spo11 (Rec12)-initiated recombination in the genome. Recombination occurs within and is regulated in part by chromatin structure, but relatively few of the many chromatin remodeling factors and histone posttranslational modifications (PTMs) have been interrogated for a role in the process.

Results

We developed a chromatin affinity purification and mass spectrometry-based approach to identify proteins and histone PTMs that regulate recombination hotspots. Small (4.2 kbp) minichromosomes (MiniCs) bearing the fission yeast ade6-M26 hotspot or a basal recombination control were purified approximately 100,000-fold under native conditions from meiosis; then, associated proteins and histone PTMs wer

SUBMITTER: Storey AJ 

PROVIDER: S-EPMC6205778 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature