Correction-free force calibration for magnetic tweezers experiments.

ABSTRACT: Magnetic tweezers are a powerful technique to perform high-throughput and high-resolution force spectroscopy experiments at the single-molecule level. The camera-based detection of magnetic tweezers enables the observation of hundreds of magnetic beads in parallel, and therefore the characterization of the mechanochemical behavior of hundreds of nucleic acids and enzymes. However, magnetic tweezers experiments require an accurate force calibration to extract quantitative data, which is limited to low forces if the deleterious effect of the finite camera open shutter time (τ_sh) is not corrected. Here, we provide a simple method to perform correction-free force calibration for high-throughput magnetic tweezers at low image acquisition frequency (f_ac). By significantly r

SUBMITTER: Ostrofet E 

PROVIDER: S-EPMC6206022 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature