An atypical RNA silencing suppression strategy provides a snapshot of the evolution of sweet potato-infecting potyviruses.

ABSTRACT: Plant viruses usually encode proteins with RNA silencing suppression (RSS) activity to counteract plant defenses. In Potyvirus, the largest genus in the family Potyviridae, this role is taken over by the multifunctional HCPro, also involved in aphid transmission, polyprotein processing and virion formation. Recently, the large P1 of Sweet potato feathery mottle virus (SPFMV) was characterized finding an extra ORF produced after polymerase slippage, which originates the product P1N-PISPO. Transient expression assays showed that SPFMV P1 and P1N-PISPO presented RSS activity, while HCPro did not. In this work, we analyze possible differences between HCPro of SPFMV and other potyviruses, testing HCPro RSS activity in a transient expression assay, and using a Plum pox virus-based system to test

SUBMITTER: Rodamilans B 

PROVIDER: S-EPMC6206096 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature