Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae.

ABSTRACT: The fruit fly Drosophila melanogaster is a model organism to study several aspects of metazoan biology. Most of the work has been conducted in adult fruit flies, including laboratory and field-derived specimens, but Drosophila melanogaster larvae recently became a valuable model to better understand animal physiology, development, or host-microbe interactions. While adult flies can be easily assigned to a given Drosophila species based on morphological characteristics, such visual identification is more intricate at the larval stage. This could explain the limited number of studies focusing on larvae, especially field-derived samples. Here, we developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay that discriminates D. melano

SUBMITTER: Raquin V 

PROVIDER: S-EPMC6206224 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature