Idiopathic male infertility is strongly associated with aberrant DNA methylation of imprinted loci in sperm: a case-control study.

ABSTRACT:

Background

Male infertility is a complex disease caused by a combination of genetic, environmental, and lifestyle factors. Abnormal epigenetic programming has been proposed as a possible mechanism compromising male fertility. Recent studies suggest that aberrant imprinting in spermatozoa in a subset of infertile men is a risk factor for congenital diseases in children conceived via assisted reproduction techniques. In this study, we examined the DNA methylation status of CpG sites within the differentially methylated regions (DMRs) of three imprinted genes, H19, GNAS, and DIRAS3, using combined bisulfite PCR restriction analysis and bisulfite sequencing in sperm obtained from 135 men with idiopathic male infertility, including normozoospermia (n = 39), moderate oligozoospermia (n =

SUBMITTER: Tang Q 

PROVIDER: S-EPMC6206675 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature