An important role for periplasmic storage in Pseudomonas aeruginosa copper homeostasis revealed by a combined experimental and computational modeling study.

ABSTRACT: Biological systems require precise copper homeostasis enabling metallation of cuproproteins while preventing metal toxicity. In bacteria, sensing, transport, and storage molecules act in coordination to fulfill these roles. However, there is not yet a kinetic schema explaining the system integration. Here, we report a model emerging from experimental and computational approaches that describes the dynamics of copper distribution in Pseudomonas aeruginosa. Based on copper uptake experiments, a minimal kinetic model describes well the copper distribution in the wild-type bacteria but is unable to explain the behavior of the mutant strain lacking CopA1, a key Cu⁺ efflux ATPase. The model was expanded through an iterative hypothesis-driven approach, arriving to a mechanism that cons

SUBMITTER: Parmar JH 

PROVIDER: S-EPMC6207460 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature