In vivo two-photon imaging of the embryonic cortex reveals spontaneous ketamine-sensitive calcium activity.

ABSTRACT: Prior to sensory experience spontaneous activity appears to play a fundamental role in the correct formation of prominent functional features of different cortical regions. The use of anaesthesia during pregnancy such as ketamine is largely considered to negatively affect neuronal development by interfering with synaptic transmission. Interestingly, the characteristics of spontaneous activity as well as the acute functional effects of maternal anaesthesia remain largely untested in the embryonic cortex in vivo. In the present work, we performed in vivo imaging of spontaneous calcium activity and cell motility in the marginal zone of the cortex of E14-15 embryos connected to the mother. We made use of a preparation where the blood circulation from the mother through the umbilical cord is pr

SUBMITTER: Yuryev M 

PROVIDER: S-EPMC6207746 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature