Identifying deer antler uhrf1 proliferation and s100a10 mineralization genes using comparative RNA-seq.

ABSTRACT:

Background

Deer antlers are bony structures that re-grow at very high rates, making them an attractive model for studying rapid bone regeneration.

Methods

To identify the genes that are involved in this fast pace of bone growth, an in vitro RNA-seq model that paralleled the sharp differences in bone growth between deer antlers and humans was established. Subsequently, RNA-seq (> 60 million reads per library) was used to compare transcriptomic profiles. Uniquely expressed deer antler proliferation as well as mineralization genes were identified via a combination of differential gene expression and subtraction analysis. Thereafter, the physiological relevance as well as contributions of these identified genes were determined by immunofluorescence, gene overexpression, and gene

SUBMITTER: Ker DFE 

PROVIDER: S-EPMC6208050 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature