An advanced enrichment method for rare somatic retroelement insertions sequencing.

ABSTRACT:

Background

There is increasing evidence that the transpositional activity of retroelements (REs) is not limited to germ line cells, but often occurs in tumor and normal somatic cells. Somatic transpositions were found in several human tissues and are especially typical for the brain. Several computational and experimental approaches for detection of somatic retroelement insertions was developed in the past few years. These approaches were successfully applied to detect somatic insertions in clonally expanded tumor cells. At the same time, identification of somatic insertions presented in small proportion of cells, such as neurons, remains a considerable challenge.

Results

In this study, we developed a normalization procedure for library enrichment by DNA sequences correspond

SUBMITTER: Komkov AY 

PROVIDER: S-EPMC6208084 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature