Heterogeneous expression of EPCAM in human circulating tumour cells from patient-derived xenografts.

ABSTRACT:

Background

We aim to characterize the heterogeneous circulating tumour cells (CTCs) in peripheral blood, independently of physical or immunological purification, by using patient-derived xenografts (PDXs) models. CTC studies from blood generally rely on enrichment or purification. Conversely, we devised a method for the inclusive study of human cells from blood of PDX models, without pre-selection or enrichment.

Methods

A qRT-PCR assay was developed to detect human and cancer-related transcripts from CTCs in PDXs. We quantified the EPCAM and keratins CTC markers, in a PDX cohort of breast cancer. The murine beta actin gene was used for normalization. Spearman's rho coefficients were calculated for correlation.

Results

We demonstrated, for the first time, that we can q

SUBMITTER: Agnoletto C 

PROVIDER: S-EPMC6208170 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature