Lens-free microscopy for 3D + time acquisitions of 3D cell culture.

ABSTRACT: Thanks to a novel three-dimensional imaging platform based on lens-free microscopy, it is possible to perform multi-angle acquisitions and holographic reconstructions of 3D cell cultures directly into the incubator. Being able of reconstructing volumes as large as ~5 mm³ over a period of time covering several days, allows us to observe a broad range of migration strategies only present in 3D environment, whether it is single cell migration, collective migrations of cells and dispersal of cells. In addition we are able to distinguish new interesting phenomena, e.g. large-scale cell-to-matrix interactions (>1 mm), fusion of cell clusters into large aggregate (~10,000 µm²) and conversely, total dissociation of cell clusters into clumps of migrating cells. This work on a

SUBMITTER: Berdeu A 

PROVIDER: S-EPMC6208343 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature