Transcriptionally Active Lung Microbiome and Its Association with Bacterial Biomass and Host Inflammatory Status.

ABSTRACT: Alteration of the lung microbiome has been observed in several respiratory tract diseases. However, most previous studies were based on 16S ribosomal RNA and shotgun metagenome sequencing; the viability and functional activity of the microbiome, as well as its interaction with host immune systems, have not been well studied. To characterize the active lung microbiome and its associations with host immune response and clinical features, we applied metatranscriptome sequencing to bronchoalveolar lavage fluid (BALF) samples from 25 patients with chronic obstructive pulmonary disease (COPD) and from nine control cases without known pulmonary disease. Community structure analyses revealed three distinct microbial compositions, which were significantly correlated with bacterial biomass, human Th

SUBMITTER: Ren L 

PROVIDER: S-EPMC6208642 | biostudies-literature | 2018 Sep-Oct

REPOSITORIES: biostudies-literature