Harmonization of exosome isolation from culture supernatants for optimized proteomics analysis.

ABSTRACT: Exosomes, the smallest subset of extracellular vesicles (EVs), have recently attracted much attention in the scientific community. Their involvement in intercellular communication and molecular reprogramming of different cell types created a demand for a stringent characterization of the proteome which exosomes carry and deliver to recipient cells. Mass spectrometry (MS) has been extensively used for exosome protein profiling. Unfortunately, no standards have been established for exosome isolation and their preparation for MS, leading to accumulation of artefactual data. These include the presence of high-abundance exosome-contaminating serum proteins in culture media which mask low-abundance exosome-specific components, isolation methods that fail to yield "pure" vesicles or variability i

SUBMITTER: Abramowicz A 

PROVIDER: S-EPMC6209201 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature