Neuraminidase-mediated desialylation augments AAV9-mediated gene expression in skeletal muscle.

ABSTRACT:

Background

Following systemic delivery, AAV9-mediated gene expression is significantly increased in ischemic versus non-ischemic muscle, suggesting that AAV9 is an attractive vector for treating peripheral arterial disease. Potential mechanisms underlying ischemia-augmented expression include: (i) increased vascular permeability and (ii) "unmasking" of endogenous AAV9 receptors. In the present study, we aimed to reconstitute the ischemic induction of AAV9 in vivo, using local injection of histamine (to increase vascular permeability) and neuraminidase (to desialylate cell surface glycans).

Methods

Bioassays were performed to optimize the effects of histamine and neuraminidase after intramuscular injection. Histamine and/or neuraminidase were then injected intramuscularly sho

SUBMITTER: Zhu H 

PROVIDER: S-EPMC6209320 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature