Myosin V functions as a vesicle tether at the plasma membrane to control neurotransmitter release in central synapses.

ABSTRACT: Synaptic vesicle fusion occurs at specialized release sites at the active zone. How refilling of release sites with new vesicles is regulated in central synapses remains poorly understood. Using nanoscale-resolution detection of individual release events in rat hippocampal synapses we found that inhibition of myosin V, the predominant vesicle-associated motor, strongly reduced refilling of the release sites during repetitive stimulation. Single-vesicle tracking revealed that recycling vesicles continuously shuttle between a plasma membrane pool and an inner pool. Vesicle retention at the membrane pool was regulated by neural activity in a myosin V dependent manner. Ultrastructural measurements of vesicle occupancy at the plasma membrane together with analyses of single-vesicle trajectories

SUBMITTER: Maschi D 

PROVIDER: S-EPMC6209431 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature