Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis.

ABSTRACT: Both mitochondria and lysosomes are essential for maintaining cellular homeostasis, and dysfunction of both organelles has been observed in multiple diseases. Mitochondria are highly dynamic and undergo fission and fusion to maintain a functional mitochondrial network, which drives cellular metabolism. Lysosomes similarly undergo constant dynamic regulation by the RAB7 GTPase, which cycles from an active GTP-bound state into an inactive GDP-bound state upon GTP hydrolysis. Here we have identified the formation and regulation of mitochondria-lysosome membrane contact sites using electron microscopy, structured illumination microscopy and high spatial and temporal resolution confocal live cell imaging. Mitochondria-lysosome contacts formed dynamically in healthy untreated cells and were dist

SUBMITTER: Wong YC 

PROVIDER: S-EPMC6209448 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature