Multiple polycomb epigenetic regulatory proteins are active in normal and regenerating adult olfactory epithelium.

ABSTRACT:

Objectives

To investigate epigenetic mechanisms contributing to regulation of cellular renewal and neurogenesis in adult olfactory epithelium (OE).

Study design

Prospective basic science study.

Methods

Olfactory basal cell cultures were prepared from adult mice per established protocols. in vivo studies were performed using the mouse methimazole lesion-regeneration paradigm. Nasal tissue sections were prepared from adult mice 7 days following lesion, or from unlesioned controls. Polycomb proteins were assessed by Western blot from culture or nasal tissue lysates, and by gene expression studies from cultures. In addition, in vivo expression patterns of Polycomb proteins were examined using immunohistochemistry. Chromosome immunoprecipitation (ChIP) was performed to inv

SUBMITTER: Goldstein BJ 

PROVIDER: S-EPMC6209616 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature