Characterization and partial purification of an antibacterial agent from halophilic actinomycetes Kocuria sp. strain rsk4.

ABSTRACT: Introduction : The inevitable rise of antibiotic-resistant bacteria is a global health problem. These pathogens erode the utility of available antibiotics. Staphylococcus aureus is one of the major causes of community-acquired infections. The aim of work was to evaluate the marine actinomycetes for production of the antibacterial agent against pathogens. Methods : Halophilic actinomycetes were isolated, characterized and screened for production of antibacterial agent against pathogenic bacteria. The antibacterial compounds were extracted by solvent extraction and separated by TLC based bioautography. Antibacterial compound was further purified by flash chromatography followed by high-performance liquid chromatography (HPLC) techniques. The active fraction

SUBMITTER: Kumar RR 

PROVIDER: S-EPMC6209832 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature