RNA Aptamers with Specificity for Heparosan and Chondroitin Glycosaminoglycans.

ABSTRACT: In this study, two respective groups of RNA aptamers have been selected against two main classes of glycosaminoglycans (GAGs), heparosan, and chondroitin, as they have proven difficult to specifically detect in biological samples. GAGs are linear, anionic, polydisperse polysaccharides found ubiquitously in nature, yet their detection remains problematic. GAGs comprised repeating disaccharide units, consisting of uronic acid and hexosamine residues that are often also sulfated at various positions. Monoclonal antibodies are frequently used in biology and medicine to recognize various biological analytes with high affinity and specificity. However, GAGs are conserved across the whole animal phylogenic tree and are nonimmunogenic in hosts traditionally used for natural antibody generation. Th

SUBMITTER: Kizer M 

PROVIDER: S-EPMC6210061 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature