The Complete Pathway for Thiosulfate Utilization in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae is known to grow with thiosulfate as a sulfur source, and it produces more ethanol when using thiosulfate than using sulfate. Here, we report how it assimilates thiosulfate. S. cerevisiae absorbed thiosulfate into the cell through two sulfate permeases, Sul1 and Sul2. Two rhodaneses, Rdl1 and Rdl2, converted thiosulfate to a persulfide and sulfite. The persulfide was reduced by cellular thiols to H₂S, and sulfite was reduced by sulfite reductase to H₂S. Cysteine synthase incorporated H₂S into O-acetyl-l-homoserine to produce l-homocysteine, which is the precursor for cysteine and methionine in S. cerevisiae Several other rhodaneses replaced Rdl1 and Rdl2 for thiosulfate utilization in the yeast. Thus, any

SUBMITTER: Chen Z 

PROVIDER: S-EPMC6210100 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature