Metagenome Analysis as a Tool to Study Bacterial Infection Associated with Acute Surgical Abdomen.

ABSTRACT:

Background

The purpose of this study was to profile the bacterium in the ascites and blood of patients with acute surgical abdomen by metagenome analysis.

Methods

A total of 97 patients with acute surgical abdomen were included in this study. Accompanied with the standard culture procedures, ascites and blood samples were collected for metagenome analysis to measure the relative abundance of bacteria among groups of patients and between blood and ascites.

Results

Metagenomic analysis identified 107 bacterial taxa from the ascites of patients. A principal component analysis (PCA) could separate the bacteria of ascites into roughly three groups: peptic ulcer, perforated or non-perforated appendicitis, and a group which included cholecystitis, small bowel lesion, and col

SUBMITTER: Wu SC 

PROVIDER: S-EPMC6210133 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature