Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.

ABSTRACT: Glycerol is a major byproduct of biodiesel production, and enzymes that oxidize this compound have been long sought after. The recently described alcohol oxidase from the white-rot basidiomycete Phanerochaete chrysosporium (PcAOX) was reported to feature very mild activity on glycerol. Here, we describe the comprehensive structural and biochemical characterization of this enzyme. PcAOX was expressed in Escherichia coli in high yields and displayed high thermostability. Steady-state kinetics revealed that PcAOX is highly active toward methanol, ethanol, and 1-propanol ( k_cat = 18, 19, and 11 s^-1, respectively), but showed very limited activity toward glycerol ( k_obs = 0.2 s^-1 at 2 M substrate). The crystal structure of the homo-octameric PcAOX was

SUBMITTER: Nguyen QT 

PROVIDER: S-EPMC6210165 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature