Intracellular oxygen mapping using a myoglobin-mCherry probe with fluorescence lifetime imaging.

ABSTRACT: Oxygen (O2) is one of the most important biometabolites. In abundance, it serves as the limiting terminus of aerobic respiratory chains in the mitochondria of higher organisms; in deficit, it is a potent determinant of development and regulation of other physiological and therapeutic processes. Most knowledge on intracellular and interstitial concentration ([O2]) is derived from mitochondria isolated from cells or tissue biopsies, providing detailed but nonnative insight into respiratory chain function. The possible loss of essential metabolites during isolation and disruption of the normal interactions of the organelle with the cytoskeleton may cause these data to misrepresent intact cells. Several optical methodologies were also developed, but they are often unable to detect heterogeneit

SUBMITTER: Penjweini R 

PROVIDER: S-EPMC6210794 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature