Comprehensive shotgun lipidomics of human meibomian gland secretions using MS/MS^all with successive switching between acquisition polarity modes.

ABSTRACT: The lipid composition of human meibomian gland secretions (meibum) has been analyzed using both targeted and untargeted mass spectrometric approaches, each of which has its advantages and disadvantages. Herein we report the results of shotgun lipidomic profiling of human meibum using a new approach that combines the advantages of targeted and untargeted analyses to yield highly sensitive and comprehensive profiles. Samples containing an estimated 7-13 µg (8-16 nL) of human meibum lipids were analyzed using MS/MS^all, an untargeted approach for MS/MS. Using MS/MS^all with ESI and successive polarity switching, we obtained tandem mass spectra in both modes at every 1 Da step for all ions in the m/z 200-1,200 range. In approximately 12 min, a total of 2 MS sp

SUBMITTER: Chen J 

PROVIDER: S-EPMC6210907 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature