Development of an Ion Mobility Spectrometry-Orbitrap Mass Spectrometer Platform.

ABSTRACT: Complex samples benefit from multidimensional measurements where higher resolution enables more complete characterization of biological and environmental systems. To address this challenge, we developed a drift tube-based ion mobility spectrometry-Orbitrap mass spectrometer (IMS-Orbitrap MS) platform. To circumvent the time scale disparity between the fast IMS separation and the much slower Orbitrap MS acquisition, we utilized a dual gate and pseudorandom sequences to multiplex the injection of ions and allow operation in signal averaging (SA), single multiplexing (SM), and double multiplexing (DM) IMS modes to optimize the signal-to-noise ratio of the measurements. For the SM measurements, a previously developed algorithm was used to reconstruct the IMS data. A new algorithm was developed

SUBMITTER: Ibrahim YM 

PROVIDER: S-EPMC6211177 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature